direct elisa

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The enzyme-linked immunosorbent assay (ELISA) is one of the most commonly used labeled immunoassay techniques. It is based on an enzyme-labeled antibody capable of detecting an antigen immobilized to a solid surface, 96-well or 384-well polystyrene plates. A substrate is added to produce either a color change or light signal correlating to the amount of the antigen which presents in the original sample.

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